Phenotypic screening to accelerate drug discovery

Read more about using iPSC-derived somatic cells for drug discovery in Nature Reviews :

Stem-cell discovery platforms yield first clinical candidates

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Why use PhenoTest for in vitro assays ?

  • Homogeneous cell batches for large scale assays: PCi-cells are produced in large batches (> 1 bn cell) of constant quality from a given hiPSC source.
  • Access to specific genetic backgrounds: any of our PhenoTest is customizable and can be performed on PCi-cells from patient specific hiPSC lines.
  • Highly skilled and dedicated team: we are entirely committed to the success of your project and strive to achieve the highest standard of client satisfaction (please visit our feedback page).

Improving the relevance of cellular tools greatly improves drug screening efficacy and speeds-up the whole process, resulting in reduced development costs and more reliable  drugs. 

To optimize the relevance of biological information obtained in vitro, we develop cell-based assays specifically designed for target identification and validation, high throughput and high content screening, and A.D.M.E.-Tox studies.

Our assays are designed to monitor crucial physiological functions and more specific aspects of cell-type specific functions.

Shift from target-based to phenotypic assays with PhenoTests. 

Strong confidentiality and intellectual property protection.


Browse the PhenoTests catalog

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Reference Assay Endpoint Method Stimulating factor or inducer Physio-pathology
PT-MEL_LCC Live cell counting Nb of live/dead cells Calcein AM/ Annexin V fluorescent staining Basal Cell proliferation / Cell survival
PT-MEL_CRX CellROX Cell Ratio and intensity of staining Fluorescent staining Basal, paraquat Oxidative stress response, at the cell level
PT-MEL_MSX MitoSOX Cell Ratio and intensity of staining Fluorescent staining Basal, paraquat Oxidative stress response, at the mitochondrial level
PT-MEL_TUN TUNNEL Cell Ratio and intensity of staining Fluorescent staining Basal, paraquat Cell apoptosis
PT-MEL_MEL Melanin Concentration in melanin OD475 reading by photometry a-MSH Pigmentation
PT-MEL_TYR Tyrosinase activity Cell ratio and intensity of staining Fluorescence imaging Basal, Kojic acid Pigmentation
PT-MEL_PRM protein expression MITF immunolabeling Immuno-fluorescence Basal Key transcription factor, melanogenesis
PT-MEL_PAX protein expression PAX3 immunolabeling Immuno-fluorescence Basal Key transcription factor
PT-MEL_PRP protein expression PMEL17 immunolabeling Immuno-fluorescence Basal Pigmentation
PT-MEL_PRT protein expression TYRP1 immunolabeling Immuno-fluorescence Basal Pigmentation
PT-MEL_RNA mRNA expression Relative expression level for any marker RT-qPCR Basal Pigmentation disorder, melanogenesis pathway

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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Reference Assay Endpoint Method Stimulating factor or inducer Physio-pathology
PT-RPE_LCC Live cell counting Nb of live/dead cells Calcein AM/ Annexin V fluorescent staining Basal Cell proliferation / Cell survival
PT-RPE_CRX CellROX Cell Ratio and intensity of staining Fluorescent staining Basal, paraquat Oxidative stress response, at the cell level
PT-RPE_MSX MitoSOX Cell Ratio and intensity of staining Fluorescent staining Basal, Paraquat Oxidative stress response at mitochondrial level
PT-RPE_TUN TUNNEL Cell Ratio and intensity of staining Fluorescent staining Basal, paraquat Cell apoptosis
PT-RPE_MEL Melanin determination Concentration in melanin OD475 reading by photometry Basal Pigmentation
PT-RPE_PHA pH-rhodo bioparticles Cell ratio and intensity of staining Fluorescence imaging Basal Phagocytosis, retinal disorders
PT-RPE_ELV ELISA VEGF concentration OD reading by photometry Basal Growth factor secretion, retinal disorders
PT-RPE_ELP ELISA PEDF concentration OD reading by photometry Basal Growth factor secretion, retinal disorders
PT-RPE_TER Transepithelial electrical resistance Resistance in Ohm/cm2 Epithelial Volt/Ohm meter Basal Epithelial barrier, retinal disorders
PT-RPE_PRM Protein expression MITF immunolabeling Immuno-fluorescence Basal Key transcription factor
PT-RPE_PRZ Protein expression ZO-1 immunolabeling Immuno-fluorescence Basal Tight-junction, epithelial barrier
PT-RPE_PRP Protein expression PMEL17 immunolabeling Immuno-fluorescence Basal Pigmentation
PT-RPE_PRT Protein expression TYRP1 immunolabeling Immuno-fluorescence Basal Pigmentation
PT-RPE_RNA mRNA expression Relative expression level for any marker RT-qPCR Basal Retinal disorders

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 Reference  Assay  Endpoint  Method  Stimulating factor/inducer  Physiopathology
 PT-SEB_LCC  Live cell count Nb live/dead cells Calcin AM/Annexin V  Basal, Paraquat  Cell proliferation/Survival
 PT-SEB_CRX  CellROX Cell ratio and intensity of staining Fluorescent staining  Basal, Paraquat  Oxidative stress response at cell level
PT-SEB_MSX  MitoSOX Cell ratio and intensity of staining Fluorescent staining  Basal, Paraquat  Oxidative stress response at mitochondria level
 PT-SEB_TUN  TUNNEL Cell ratio and intensity of staining  Fluorescent staining  Basal, Paraquat  Apoptosis
 PT-SEB_ARS  Bodipy staining Cell ratio and intensity of staining  Fluorescent staining  Basal, testosterone or DHT, finasteride Androgen receptor stimulation, sebogenesis, acne
 PT-SEB_LAS  Bodipy staining  Cell ratio and intensity of staining  Fluorescent staining Basal, Linoleic acid  PPAR stimulation, sebogenesis
 PT-SEB_AAS  Bodipy staining Cell ratio and intensity of staining  Fluorescent staining Basal, Arachidonic acid  PPAR stimulation, sebogenesis
 PT-SEB_LCA  Lipid composition analysis Lipid composition, quantification GC/MS  Basal  Sebogenesis, acne
PT-SEB_RNA mRNA expression Relative expression level RT-qPCR Basal Sebogenesis, acne