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iPSC are derived from blood samples or fibroblasts from patients with rare diseases. We use a state-of-the-art non-integrative technique and feeder-free culture with defined medium.

 

 

For each cell line, we create a Master Cell Bank and provide a detailed, illustrated protocol for iPSC culture together with comprehensive quality control data that include:

– Pluripotency genes expression (qPCR, immunohistochemistry and FACS)

– Karyotype

– Ability to differentiate: Characterized embryoid bodies with RT-qPCR examination of the presence of derivatives of the three germ layers.