iPSC are derived from blood samples or fibroblasts from patients with rare diseases. We use a state-of-the-art non-integrative technique and feeder-free culture with defined medium.
For each cell line, we create a Master Cell Bank and provide a detailed, illustrated protocol for iPSC culture together with comprehensive quality control data that include:
– Pluripotency genes expression (qPCR, immunohistochemistry and FACS)
– Ability to differentiate: Characterized embryoid bodies with RT-qPCR examination of the presence of derivatives of the three germ layers.